Structure of the pre-initiation complex explains CMGE biogenesis
Protein expression and purification HpaII methyltransferase (MH), ORC, Cdc6, Mcm2–7–Cdt1, DDK, CDK, (yeast-expressed) Sld3/7, Cdc45, GINS, (yeast-expressed) Pol ε, Mcm10, RPA, topoisomerase I (TopoI), Pol α and Rad53 were expressed and purified as previously described24,30,40,46,66,67,68,69. All mutant constructs were expressed and purified following the same protocol as was used for the wild-type protein unless stated otherwise. … Read more